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Overview of the Nutrient Density in Beef Study

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Welcome to the Bionutrient Institute’s “Nutrient Density in Beef” Notebook, a comprehensive resource documenting the organization’s Beef Study. The notebook is a tool for hosting nutrition and food metadata for the purposes of communication, research, and development; it’s not intended to replace a published academic study.

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Purpose of the Study

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This study, spearheaded by the Bionutrient Institute, represents a pioneering effort to define nutrient density in beef. Building on extensive research into nutrient variability in crops, it ventures into new territory by examining how farm management practices, genetics, soil health, forage quality, and the microbiome influence beef nutrient composition. The findings aim to empower consumers, farmers, and supply chain managers with actionable insights into beef's nutritional value, facilitating informed decisions at every level of the supply chain.

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Study Leadership and Collaborators

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The study was a collaborative effort managed by the Bionutrient Institute in partnership with Edacious and Utah State University. The Bionutrient Institute oversaw participant engagement, sample collection, and communication. Utah State University’s Van Vliet lab led the majority of the analytical work, ensuring the scientific rigor of the results. Edacious contributed expertise in scientific analysis and provided data and software infrastructure to visualize and disseminate the findings.

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Objectives of the Research

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The primary objective of this initiative is to build a foundational dataset that can be leveraged to create a definition of nutrient density in beef. By analyzing a range of macronutrients, micronutrients, phytochemical compounds, soil metrics, microbiome, management practices, fodder dynamics, and human health responses, the study aims to define nutrient density in the context of these environmental and human-health factors.

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Participant Engagement and Sample Collection

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The study adopted a collaborative model that included a diverse group of participants from four continents—beef producers, researchers, organizations, and citizen scientists. Participants contributed by submitting samples of meat, soil, forage, and stool for analysis and completing detailed questionnaires on their management practices. In return, they received comprehensive reports based on their contributions.

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To ensure accuracy and consistency, participants adhered to standardized protocols for sample collection. This approach produced a robust dataset linking management practices to nutrient outcomes. Ribeye steak samples were collected from cattle raised under various management systems, such as grass-fed and feedlot-finished operations, to capture a broad range of nutrient variability.

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Soil samples were analyzed for carbon content, mineral availability, pH levels, and nitrogen content to assess environmental influences on forage and beef quality. Forage samples underwent mineral and phenolic profiling to evaluate feed quality. Cattle stool samples were subjected to 16S RNA sequencing to examine gut microbiome profiles and their role in nutrient absorption, as well as to identify the range of species in the animals' diets.

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What’s In the Notebook? Nutritional Analysis and Measurement Techniques

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The notebook includes a limited set of data collected during the study. It currently includes nutrition, breed, pasture, and production data but excludes soil, forage, and stool data as this data is still being synthesized and compiled by the Institute. The full study data is expected to be compiled and available to research partners early in 2025.

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The study analyzed a variety of nutrients, including proximates (total fat, total protein), fatty acids, minerals, and phytochemicals. These were reported as concentration units. Utah State continues to refine its analysis to include profiles of fat-soluble vitamins (A, D, E) and B vitamins, with results expected in early 2025.

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Initially, the study employed a metabolomics approach to assess relative nutrient abundance but later transitioned to a targeted analysis to determine absolute concentrations. This shift allowed for a better understanding of how nutrient density impacts human health by putting nutrient concentrations in the context of nutrient requirements.

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Preliminary Findings

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The study, while currently on-going, revealed significant variability in beef nutrient density, driven by diverse production factors. Early findings are showing that beef from cattle raised on diverse pastures exhibited lower omega-6 to omega-3 ratios and higher phytochemical richness compared to beef from monoculture pastures or feedlots. Key nutrients such as secondary metabolites, fatty acids, and minerals, alongside soil and forage metrics, demonstrated the influence of management practices. These findings highlight opportunities for improving nutrient density through targeted interventions.

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Next Steps

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With the launch of the Institute’s dashboard, we are now inviting submissions to join the Bionutrient Definition Standards Board (BDSB). We seek highly knowledgeable individuals in the fields of soil agronomy, gut microbiology, nutritional biochemistry, beef cattle management, and human inflammation.\r\nSubmissions will be carefully reviewed, and selected members will contribute to analyzing individual datasets. These datasets will be used to create scaled reports ranking each component from best to worst. The findings will then be integrated into an algorithm to establish the first-ever definition of the Institute’s definition of Nutrient Density in Beef.\r\nIf you would like to nominate someone for the board—whether yourself or another individual—you are welcome to begin the process here.

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There are trace levels of cobalt in many foods, however cobalt needs are primarily met through consuming vitamin B12-containing foods.",{"_27":3290,"_2586":3291,"_35":2592},"64b89b01-6d8b-4008-bb44-3c18de2ab5b1",{"_2588":491,"_2590":3292},"Cobamamide",{"_27":3294,"_2586":3295,"_35":3297},"639aea87-2a60-4b2b-92be-54fd22d85971",{"_2588":695,"_2590":3296},"Conjugated Linoleic Acid (C18:2 trans-10, cis-12)","Trans-10, Cis-12",{"_27":3299,"_2586":3300,"_35":3302},"319bf801-06a3-4a24-8177-dd1c19ab216a",{"_2588":601,"_2590":3301},"Conjugated Linoleic Acids","Conjugated linoleic acids (CLAs) are a type of trans-fat produced by microbes in ruminant animals. CLA levels in foods can vary due to different agricultural management practices, however their impact on human health is unclear.",{"_27":3304,"_2586":3305,"_35":3307},"e6a436e3-7035-43c4-97b2-a0dc7f771155",{"_2588":743,"_2590":3306},"Copper","Copper assists in converting food into energy, managing iron levels in the body, and protecting cells from damage due to oxidative stress.",{"_27":3309,"_2586":3310,"_35":2592},"b18fee1c-4463-44e2-87bb-6a47559cbd18",{"_2588":3311,"_2590":3312},"EDA:1291","Coumestrol",{"_27":3314,"_2586":3315,"_35":2592},"2371a06e-4903-41d1-b8bd-df1429b23b31",{"_2588":775,"_2590":3316},"Total (Crude) Protein",{"_27":3318,"_2586":3319,"_35":2592},"f07dfb63-23eb-4568-ab86-33a50f30ffe3",{"_2588":3320,"_2590":3321},"EDA:1292","Cyanidin",{"_27":3323,"_2586":3324,"_35":2592},"15af094d-b247-445f-b0ed-8e2712804bb5",{"_2588":1710,"_2590":3325},"Cysteine",{"_27":3327,"_2586":3328,"_35":2592},"0bde2724-18a0-41ba-bce2-8c3fd6ad17d3",{"_2588":1245,"_2590":3329},"Cystine",{"_27":3331,"_2586":3332,"_35":2592},"299d1513-e6e9-4925-8e12-643e30b5a785",{"_2588":3333,"_2590":3334},"EDA:1286","Daidzein",{"_27":3336,"_2586":3337,"_35":2592},"ee45227e-969c-4139-84cb-46695a51979d",{"_2588":3338,"_2590":3339},"EDA:1305","Daidzin",{"_27":3341,"_2586":3342,"_35":2592},"61a130e1-cdea-4e72-950b-4f2750a03fe1",{"_2588":735,"_2590":3343},"Capric Acid (C10:0)",{"_27":3345,"_2586":3346,"_35":2592},"cb6b6988-7787-4fa2-83d9-a649f501047f",{"_2588":3347,"_2590":3348},"EDA:1293","Delphinidin",{"_27":3350,"_2586":3351,"_35":2592},"af348bf6-e3cc-4902-9b7f-d42a2cecea07",{"_2588":3352,"_2590":3353},"EDA:761","Delta-5-avenasterol",{"_27":3355,"_2586":3356,"_35":2592},"00240c15-60ef-4f84-850d-1f8857dee2c9",{"_2588":3357,"_2590":3358},"EDA:1284","Delta-7-avenasterol",{"_27":3360,"_2586":3361,"_35":2592},"58f1908d-cf93-4d03-abf8-4a57156de006",{"_2588":733,"_2590":3362},"Delta-Tocopherol",{"_27":3364,"_2586":3365,"_35":2592},"d3704b29-cfb8-4125-8117-94720745210b",{"_2588":3366,"_2590":3367},"EDA:401","delta-tocotrienol",{"_27":3369,"_2586":3370,"_35":2592},"f6217ebb-903f-444f-8355-f0045b498f41",{"_2588":1150,"_2590":3371},"Dietary Ash",{"_27":3373,"_2586":3374,"_35":2592},"f34c4d56-66b8-45c5-b781-3e6722fb4707",{"_2588":1242,"_2590":3375},"Beta-Carotene",{"_27":3377,"_2586":3378,"_35":3380},"47b6588f-c9c8-4891-9711-c1323eea361a",{"_2588":1189,"_2590":3379},"Dietary Fiber","Dietary fiber is a type of carbohydrate that is not broken down by the body. 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Not well studied outside of nutrition.",{"_27":3407,"_2586":3408,"_35":2592},"d0a007fc-7b19-4c9b-a1f7-fa749d0cd0a5",{"_2588":1898,"_2590":3409},"Dimethyl Sulfone",{"_27":3411,"_2586":3412,"_35":2592},"9c349e32-cfce-440c-a87f-0e9c8bc4e0fa",{"_2588":3413,"_2590":3414},"EDA:1310","Diosmetin",{"_27":3416,"_2586":3417,"_35":2592},"09a83741-085d-465f-a744-24670b01fe7a",{"_2588":1778,"_2590":3418},"D-Limonene",{"_27":3420,"_2586":3421,"_35":2592},"644d5da2-1eb1-4bf3-acb0-6726d28adeab",{"_2588":3422,"_2590":3423},"EDA:885","Docosadienoic (22:2 N6) Undifferentiated",{"_27":3425,"_2586":3426,"_35":2592},"d8e2cc7d-903b-4b26-89c2-8b2dd1f4cb77",{"_2588":1321,"_2590":3427},"Docosadienoic acid undifferentiated (22:2)",{"_27":3429,"_2586":3430,"_35":2592},"39abdc00-b50c-4051-85ce-98eb5a795735",{"_2588":3431,"_2590":3432},"EDA:880","Docosahexaenoic Acid (22:6 N-3) 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added",{"_27":4889,"_2586":4890,"_35":4892},"b76be7b5-a9de-4b5f-b0ee-06fc25523ed5",{"_2588":683,"_2590":4891},"B12 (Cobalamin)","Vitamin B12 (cobalamin) assists in DNA synthesis, red blood cell production, and insulation around neurons.",{"_27":4894,"_2586":4895,"_35":4897},"2c84bd3b-5171-4e90-9877-ed6e29b97455",{"_2588":805,"_2590":4896},"B1 (Thiamin)","Thiamin is a B vitamin required by cells for normal function and growth, including converting food into energy and creating building blocks for DNA.",{"_27":4899,"_2586":4900,"_35":4902},"d4e1d212-0ef9-4388-9ef8-e122dc9ee8f6",{"_2588":807,"_2590":4901},"B2 (Riboflavin)","Vitamin B2 (riboflavin) helps convert food into energy and protects cells from damage due to oxidative stress.",{"_27":4904,"_2586":4905,"_35":4907},"fd80f9bc-f85f-42e7-9b38-3e921380c67e",{"_2588":617,"_2590":4906},"B3 (Niacin)","Vitamin B3 (niacin) assists cells in converting food into energy and DNA repair.",{"_27":4909,"_2586":4910,"_35":4912},"2a4fa7ec-fa9e-4481-ad6c-a411de50214f",{"_2588":630,"_2590":4911},"B5 (Pantothenic Acid)","Vitamin B5 (pantothenic acid) assists in converting food into energy and helps cells build and break down fat.",{"_27":4914,"_2586":4915,"_35":2592},"e75348a7-8e44-434d-978c-0de2a67a5a16",{"_2588":799,"_2590":4916},"Vitamin B6 Total",{"_27":4918,"_2586":4919,"_35":4921},"112f4b58-26d9-4d33-b82e-dfe21f88ef50",{"_2588":763,"_2590":4920},"B6 (Pyridoxal)","Vitamin B6 (pyridoxal) assists in chemical reactions required for protein synthesis and red blood cell production.",{"_27":4923,"_2586":4924,"_35":4926},"479f8d66-ffa9-4326-ae8c-5367778fa28f",{"_2588":674,"_2590":4925},"B7 (Biotin)","Vitamin B7 (biotin) is required for cellular growth and function and assists in building and breaking down fats, carbohydrates, and protein.",{"_27":4928,"_2586":4929,"_35":4931},"06a529cb-f231-4333-8e3f-f2e550ebead8",{"_2588":597,"_2590":4930},"B9 (Folate)","Vitamin B9 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